〔Abstract〕 Objective To study anti-hepatitis C virus NS3/4A protease activity ofLonicera fulvotomentosabased on fluorescence method and molecular docking. Methods The activity of HCV NS3/4A protease inhibition ofL.fulvotomentosaextracts was tested by fluorescence method.The binding of the main active ingredients to HCV NS3/4A virus protease was analyzed based on molecular docking. Results The water extract and alcohol extract ofL.fulvotomentosahad a good inhibitory effect on the activity of HCV NS3/4A protease with IC50of 0.005-0.019 mg/ml.Based on molecular coupling and interaction analysis, it was found that chlorogenic acid, isochlorogenic acid A,cynaroside and luteolin combined well with HCV NS3/4A protease, forming multiple hydrogen bonds. Conclusion The active components ofL.fulvotomentosaagainst HCV NS3/4A protease activity were isochlorogenic acid A and luteolin.