〔Abstract〕 Objective To explore the changing characteristics of Wnt2/β-catenin pathway in liver regeneration and repair in C57BL/6 mice. Methods Male C57BL/6 mice were randomly divided into a sham-operated group(Sham group),1 d post-operative group, 2 d post-operative group, 4 d post-operative group, 6 d post-operative group, and 8 d post-operative group.Mice in the operated group underwent partial hepatectomy(PHx) to remove the left and middle lobes of the liver, respectively.Plasma and liver tissues were collected on postoperative days 1,2,4,6,and 8,and plasma ALT and AST activity was measured by ALT(alanine aminotransferase) and AST(aspartate aminotransferase) biochemical analysis kits; Ki67-positive cells were identified by immunohistochemistry in each group; the number of HNF4-α and Ki67 double positive cells, the number of LYVE1 and Ki67 double positive cells, and the number of β-catenin transferred into the nucleus were determined by immunofluorescence method.The expression of Wnt2 protein in each group was detected by Western blot, and the time characteristics of its expression during liver regeneration were analyzed. Results Liver weight and liver/body weight ratio peaked on day 6 after PHx and approached the level of the Sham group.After PHx, there was severe liver damage on day 1,but it had normalized by day 4.On day 2 after PHx, mainly hepatocytes proliferated; and on days 4 and 6,mainly liver sinusoidal endothelial cells proliferated, while the Wnt2/β-catenin pathway was activated. Conclusion The liver has a powerful regenerative repair ability, which is closely related to the rapid proliferation of hepatocytes and liver sinusoidal endothelial cells, and the activation of the Wnt2/β-catenin pathway is activated in the regenerative repair of the mouse liver.