〔Abstract〕 Objective To investigate the effects of dihydroartemisinin(DHA) on cell growth, migration invasion and vasculogenic mimicry ability of non-small cell lung cancer(NSCLC). Methods Different concentrations of DHA were added to NSCLC cell lines A549 and H3255,and after 24 hours, the cell viability was detected by CCK8 method, and the migration and invasion ability of NSCLC cells was detected by Transwell experiment.qRT-PCR and Western blot detected E-cadherin, N-cadherin, and Vimentin mRNA and protein expression levels, respectively.Three-dimensional cell was cultured to observe the vascular-like morphological generation of cells.qRT-PCR and Western blot detected human vascular endothelial cadherin(VE-cadherin) mRNA and protein expression levels as marker of vasculogenic mimicry. Results DHA inhibited cell growth of A549 and H3255 and showed time-dependent and concentration-dependent.DHA inhibits the invasion and metastasis(allP<0.001) of A549 and H3255 cells; DHA upregulated the expression of E-Cadherin at mRNA(allP<0.001) and protein(P<0.001;P<0.01) levels in A549 and H3255 cells, and downregulated the expression of N-cadherin at mRNA(allP<0.01) and protein(allP<0.001) levels as well as the expression of Vimentin at mRNA(P<0.01;P<0.001) and protein(allP<0.001) levels.The results of three-dimensional cell culture showed that the 12-hour vasculogenic mimicry generation capacity of DHA-treated A549 cells and H3255 cells was reduced, and the expressions of VE-cadherin at mRNA(P<0.001;P<0.01) and protein(allP<0.001) levels were downregulated. Conclusion Dihydroartemisinin inhibits the growth, migration invasion and vasculogenic mimicry ability of non-small cell lung cancer cells.