〔Abstract〕 Objective To explore the effect of C1q/tumor necrosis factor-related protein 9(CTRP9) on the expression of genes and proteins related to lipid metabolism of brown adipose tissue(BAT) in mice after cold stimulation. Methods C57BL/6J male mice were injected with adenovirus Ad-GFP(control group) or Ad-CTRP9(experience group) into the scapular region and kept for 7 days. After cold stimulation at 4 ℃ for 10 hours, the expression levels of BAT marker genes and proteins were detected by real time PCR and Western blot. Results Overexpression of CTRP9 induced by cold stimulation significantly increased the mRNA level of iodothyronine deiodinase 2(Dio2) in BAT(P<0.01). Additionally, there was no significant difference in the expression of BAT marker genes(UCP-1,PGC-1α,PRDM16andARβ3), and liposynthesis and lipolysis related genes(PPARγ,HSLandATGL). Uncoupling protein 1(UCP-1) protein expression was upregualted in Ad-CTRP9 compared to the Ad-GFP control group, while the expression of lipolysis related protein adipose triglyceride lipase(ATGL) decreased significantly(P<0.05). Conclusion In cold environment, overexpression of CTRP9 promotes the accumulation of UCP-1 protein in BAT, upregulates the expression of thyroid hormone signal related geneDio2, and inhibits triglyceride hydrolysis to maintain a constant body temperature.