〔Abstract〕 Objective To obtain chimeric antigen receptor macrophages(CAR-M) targeting HER2 stably transfected. Methods CAR lentivirus vector targeting HER2 was constructed and infected with human monocytic leukemia cell line(THP-1). CAR THP-1 cells with green fluorescent labeling were selected by sorting flow cytometry and continued to be culturedin vitro. The CAR THP-1 cells targeting HER2 were co-cultured with the endometrial cancer cell line Ishikawa with negative and positive HER2 expression, and their targeted phagocytosis of CAR-M to HER2 positive tumor cells was detected by imaging flow cytometry, and the targeted phagocytosis efficiency of CAR-M to HER2 positive tumor cells was detected by flow cytometry. Results CAR lentivirus infection with THP-1 cells was less efficient; After co-culture with cancer cells, flow cytometry and imaging flow cytometry showed that CAR THP-1 cells had enhanced phagocytosis of HER2 positive Ishikawa cells compared with the empty body group(P<0.01). Conclusion In this experiment, CAR THP-1 cell line targeting HER2 was established by constructing CAR lentivirus vector and transfecting THP-1 cells, and it was proved that CAR THP-1 could phagocytize HER2 positive Ishikawa cells through specific targeting.