〔Abstract〕 Objective To explore the biocompatibility of Polydimethylsiloxane/Chlorhexidine gluconate(PDMS/CHG) composite coating on titanium surface. Methods CHG with different concentrations were covalently grafted to the surface of titanium through PDMS and divided into 5 groups: Group Ti: blank control group; Group P:PDMS coating group; Group C1:treated with PDMS and 0.2 mg/ml CHG;Group C2:treated with PDMS and 0.4 mg/ml CHG;Group C3:treated with PDMS and 0.8 mg/ml CHG;the surface morphology of titanium was observed with scanning electron microscope(SEM),and rat gingival fibroblasts(RGFs) were inoculated to the sample surface co-cultivation.Confocal laser scanning microscope(CLSM)was used to observe the cells, on the surface of titanium, morphology and the state of the cell after FITC-labeled phalloidin and AO/EB staining, besides, CCK-8 was used to detect cells proliferation. Results It could be seen by SEM that films had been formed on the surface of titanium sheets in each experimental group.Combined with elemental analysis, the films on the surfaces of C2 and C3 groups were uniform and dense; CLSM showed that the cell morphology of each experimental group was consistent with that of the titanium group, with better extension, and the results of AO/EB staining showed that there was a lot of green fluorescence in each group, and the cells were in good shape.CCK-8 results showed that there was no difference between each group(P>0.05). Conclusion Titanium surface combined with concentration of no more than 0.8 mg/ml PDMS/CHG coating has good biocompatibility.