〔Abstract〕 Objective To explore the effects of isogenous micro-grafting(MG) technology and the combined use of botulinum toxin type A(BTX-A) on hair regeneration in mice. Methods Forty-five C57BL/6 mice were randomly divided into five groups: normal saline group(NS group), MG group(MB-0 group), MG+low-dose BTX-A group(MB-2 group), MG+medium dose BTX-A group(MB-10 group), MG+high-dose BTX-A group(MB-50 group). The growth of hair on the backs of the mice was observed after the administration of the drugs, and the hair follicles were evaluated by hematoxylin-eosin(HE) staining on the 7th, 14th, and 21st days of the experiment, and the expression of vascular endothelial growth factor(VEGF) and β-catenin was evaluated by immunohistochemical staining on day 21. Results After treatment with micrograft and botulinum toxin type A, the skin darkening time was shortened(P<0.05), and the coverage rate of new hair increased on the 14 th day(P<0.05). Compared with the NS group, the number of hair follicles increased(P<0.05) and the expression of VEGF and β-catenin increased in each other group. Conclusion Isogenous micro-grafting technology combined with botulinum toxin type A has a promotional effect on hair regeneration in mice, and the mechanism may be related to the promotion of vascular growth and activation of β-catenin signaling.