〔Abstract〕 Objective To investigate the expression of miR-142-5p in oral squamous cell carcinoma(OSCC) tissues and cell lines and its effects on oral squamous cell proliferation, migration, invasion and angiogenesis. Methods Sixteen groups of oral tumour tissues and paraneoplastic tissues were collected, and qRT-PCR was applied to detect the expression of miR-142-5p in the tissues. The effects of miR-142-5p on cell proliferation, migration, andinvasion were observed by cell counting kit-8(CCK-8), cloning, wound healing, Transwell, invasion assays, and the effect of miR-142-5p on angiogenesis was also detected by lumen formation assay.The expression of angiogenesisrelated proteins vascular endothelial growth factor(VEGFA), vascular endothelial calreticulin(VE-cadherin), epithelial calreticulin(E-cadherin), matrix metalloproteinase 2(MMP2), and matrix metalloproteinase 9(MMP9) was detected by Western blot after overexpression of miR-142-5p. Results miR-142-5p was lowly expressed in oral tumour tissues and cell lines. CCK-8 and clonogenic assays showed that miR-142-5p was inversely correlated with the proliferation of OSCC cells, wound healing and Transwell assays showed that miR-142-5p was inversely correlated with the migration of OSCC cells, and cell invasion assays showed that miR-142-5p was conversely correlated with the invasion of OSCC cells. Analysis of lumen formation assay showed that overexpression of miR-142-5p reduced the tube length and nodes of HUVECs. Western blot assay showed that up-regulation of miR-142-5p inhibited the VEGFA, VE-cadherin, MMP2, MMP9 expression and promoted E-cadherin expression. Conclusion Overexpression of miR-142-5p inhibites the proliferative, migratory and invasive effects of oral squamous carcinoma cells as well as angiogenesis, suggesting that miR-142-5p is a novel target for anti-tumour angiogenesis and against oral squamous carcinoma.