〔Abstract〕 Objective To isolate aAcinetobacter baumannii(Ab) phage from underground sewage, study its properties, and to provide a theoretical basis for phage treatment of Ab infection. Methods Double-layer agar technique was used to isolate phages by using Ab GY-6 as the host strain. Biological characterization and therapeutic effect of the phage was tested. Genetic information of the phage was analyzed. Results Ab phage Abgy202162 was isolated. Transmission electron microscopy(TEM) analysis showed that the morphology of Abgy202162 exhibited an icosahedral structure. Biological characteristic analysis showed that the optimal multiplicity of infection was 1, the latent period was 5 min, and the burst size was approximately 520 PFU per cell. In addition, Abgy202162 remained stable at different concentrations of chloroform, pH, and temperatures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that it contained 10 proteins with molecular weights ranging from 15 to 100 ku. The double-stranded(ds) DNA genome of Abgy202162 consisted of 40 889 bp and its G+C content was 38.85%. It contained 47 open reading frames(ORFs), of which 26 had specific functions, but no virulence related genes or antibiotic resistance genes were found. Phylogenetic analysis showed that Abgy202162 was a new phage in the Autographiviridae family, Beijerinkvirinae subfamily, and Friunavirus genus. Abgy202162 showed the ability to prevent Ab infection in the Galleria mellonellain vivomodel. Conclusion The phage Abgy202162 has strong environmental tolerance and high safety, indicating its potential as an antibiotic alternative used in the treatment of infections caused by Ab.