〔Abstract〕 Objective To investigate the difference between imaging flow cytometry and immunofluorescence techniques for detecting Ahr entry in fibroblast-like synoviocytes. Methods Human-derived fibroblast-like synoviocytes line MH7A was cultured with 15% DMEM. The nucleation levels of Ahr in MH7A were detected by laser scanning confocal microscopy and quantitative imaging flow cytometry(ImageStreamX Mark II) in the blank control group, the Kyn group, and the Kyn+CH223191 group, respectively. The results of the two assay techniques were compared using nonparametric tests-correlation of the results. Results Compared with the blank control group, the Kyn and Kyn+CH223191 groups of MH7A cells increased Ahr nucleation ability as measured by immunofluorescence technique and imaging flow cytometry(P<0.05), and the three groups of results calculated by the two experimental methods were consistent, with the R2 values of 0.863 8, 0.928 7, and 0.901 8, respectively, which were statistically significant differences(P<0.05). Conclusion Compared with the operation and results of immunofluorescence experiments, imaging flow cytometry data processing is more complicated. However, the results are notably precise, avoiding the subjectivity of the experimenter and minimizing experimental inaccuracies.