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Authors:Yang Luming; Hao Jinqi; Wang Lin; Pei Shengfei; Guo Yu; Tang Qinyan; Li Yue; Gao Xuelei; Li Yuhong; Hao Mingyuan; Feng Fumin
Keywords:anti-tuberculosis drug-induced liver injury;rifampicin;16S rDNA;gut microbiot;high-throughput sequencing
DOI:10.19405/j.cnki.issn1000-1492.2022.09.024
〔Abstract〕 Objective 16 S rDNA sequencing technology was used to analyze the changes of intestinal flora in rats with anti-tuberculosis drug-induced liver injury(ADLI) induced by rifampicin(RFP). Methods Twenty-four male SD rats were randomly divided into control group(D0 group),RFP gavage group for 10 days(R10 group),and RFP gavage group for 28 days(R28 group).The fecal specimens of 4 rats in each group were selected by random number table method for 16 S rDNA sequencing. Results Compared with the D0 group, the α diversity and β diversity of the R10 and R28 groups both changed(allP<0.05),the abundance ofBacteroidetesincreased at the phylum level, the abundance ofFirmicutesdecreased(allP<0.05),and the abundance ofUnclassified_f_Prevotellaceae,Blautia,Prevotellaceae_NK3B31_group,Erysipelotrichaceae_uCG-003,Fournierella,increased at the genuslevel,Lactobacillus,Romboutsia,Ruminococcaceae_uCG-014 decreased in abundance(allP<0.05). Conclusion In the process of RFP-induced ADLI in rats, the number of intestinal flora decreased, the structure and composition changed, the beneficial bacteria decreased, and the pathogenic bacteria increased, but the diversity of the flora remained unchanged.