〔Abstract〕 Abstract Objective To investigate whether syndecan-2 (SDC2) can affect the proliferation , invasion and migra- tion of cervical cancer cells by regulating ferroptosis and its possible mechanism . Methods Normal cervical epithe- lial cells H8 and cervical squamous carcinoma cells C33A were cultured and divided into H8 group and C33A group . C33A cells were cultured and divided into control group , low SDC2 expression group , SDC2 + ferroptosis inhibitor(ferrostation-1) group and SDC2 + ferroptosis inducer (erastin) group . Western blot was used to detect the protein levels of SDC2 , solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) . RT-qPCR was used to detect the SDC2 mRNA level in C33A cells . ELISA kits were used to detect the levels of re- active oxygen species (ROS) , glutathione (GSH) and ferrous ion ( Fe2 + ) in C33A cells . The cloning ability of C33A cells was detected by plate cloning. The migration ability of C33A cells was detected by scratch test. Tran- swell assay was used to detect the invasion ability of C33A cells . Results Compared with H8 group , the protein and mRNA expressions of SDC2 , SLC7A11 and GPX4 in C33A group increased (P < 0. 05) . Compared with the control group , the proliferation ability , migration ability and invasion ability of C33A cells in the low SDC2 group decreased(P < 0. 05) , the protein and mRNA expressions of SLC7A11 and GPX4 in C33A cells decreased (P < 0. 05) , and the GSH level decreased . ROS and Fe2 + levels increased (P < 0. 05) . Compared with the low SDC2 group , the protein and mRNA expressions of SLC7A11 and GPX4 increased (P < 0. 05) , the GSH level increased , and the ROS and Fe2 + levels decreased ( P < 0. 05) in the low SDC2 + ferrostation-1 group . Compared with the control group , the proliferation ability , migration ability and invasion ability of C33A cells with low SDC2 + erastin expression decreased (P < 0. 05) . Conclusion The expression of SDC2 increases in C33A cervical cancer cells . Low expression of SDC2 can activate SLC7A11 /GPX4 pathway mediated ferroptosis , thereby reducing the prolifera- tion , invasion and migration of C33A cells .