〔Abstract〕 Objective To study the mechanism of long non-coding RNA(lncRNA) RNF185-AS1 affecting the proliferation and invasion of glioma cells by regulating microRNA-637(miR-637). Methods The GEPIA database was used to analyze the expression level of RNF185-AS1 in glioma tissues and adjacent tissues. qRT-PCR was used to detect the expression level of RNF185-AS1 in glioma cell lines(SNB-19, LN382, U87 MG, U251) and normal brain astrocytes HEB. U251 cells were used as the research object, and sh-Con control plasmid(sh-Con group) and sh-RNF185-AS1 plasmid(sh-RNF185-AS1 group) were transfected respectively. qRT-PCR verified the silencing efficiency of RNF185-AS1. MTT experiment and Transwell invasion experiment were used to detect the proliferation and invasion of U251 cells after silencing RNF185-AS1. qRT-PCR and dual luciferase reporter gene experiment were used to detect the targeting relationship between RNF185-AS1 and miR-637. qRT-PCR and Western blot were used to detect the expression of high mobility group A1(HMGA1) gene. Results The expression level of RNF185-AS1 in glioma tissue was significantly higher than that in adjacent tissues(P<0.01). The expression level of RNF185-AS1 in the glioma cell line was significantly higher than that in normal brain astrocytes(P<0.01), and the expression of RNF185-AS1 in U251 cells increased the most significantly(P<0.01). Compared with the sh-Con group, the expression level of RNF185-AS1 in U251 cells in the sh-RNF185-AS1 group significantly decreased(P<0.01), the proliferation activity of U251 cells was significantly reduced(P<0.05), and the number of invaded cells was significantly less(P<0.01). RNF185-AS1 and miR-637 had complementary nucleotide sequences(P<0.01). Compared with the sh-Con group, the expression level of miR-637 in U251 cells in the sh-RNF185-AS1 group significantly increased(P<0.01), and the expression level of HMGA1 gene was significantly reduced(P<0.01). Conclusion RNF185-AS1 is highly expressed in glioma tissues and cell lines. Silencing RNF185-AS1 can inhibit the proliferation and invasion of glioma U251 cells by targeting up-regulation of miR-637 expression and down-regulation of HMGA1 gene expression.