Found programs: National Natural Science Foundation of China (No. 82102460);Graduate Student Scientific Research and Practical Innovation Project of Anhui Medical University (No. YJS20230190);Scientific Research Project of Anhui Medical University (No. 2022xkj197);Clinical Research Cultivation Program of the Second Affiliated Hospital of Anhui Medical University (No. 2021LCZD13)
Authors：Weng Shengnan; Leng Guiyun; Liu Ying; Wang Yawu; Li Xin; Yao Jie; Zhou Qiang; Tang Wei
Keywords：Lactococcus garvieae;Staphylococcus;antagonistic activity;antimicrobial active components;separation and purification;16S rDNA;oxford cup method
DOI：10.19405/j.cnki.issn1000-1492.2025.02.002
〔Abstract〕 Objective To investigate the antagonistic activity ofLactococcus garvieaeSHAMU-LG6 againstStaphylococcus. Methods VITEK 2 GP identification card, Microflex LT MALDI-TOF mass spectrometer and 16S rDNA amplification sequencing were used to identify the strain species. The antagonistic activity ofL.garvieaeSHAMU-LG6 against differentStaphylococcuswas detected by Oxford cup method for bacterial inhibition; the antimicrobial active components were preliminarily isolated and purified by adsorption on XAD16 nonionic macroporous resin, gradient ethanol elution and rotary evaporation drying. Results L.garvieaeSHAMU-LG6 exhibited potent antagonistic effect against methicillin-resistantStaphylococcus aureus, methicillin-susceptibleS.aureus,S.epidermidis,S.saprophyticus,S.lugdunensis,S.hominis,S.capitisandS.warneri, with inhibitory indices of 3.3, 3.0, 4.3, 2.0, 4.0, 3.5, 3.8, and 3.5, respectively. The antimicrobial active components produced byL.garvieaeSHAMU-LG6 were mainly present in 70% and 80% ethanol eluates. Conclusion L.garvieaeSHAMU-LG6 exhibits a potent antagonistic effect onStaphylococcus, and the antimicrobial active components produced by it are expected to be a lead compound for the development of novel antimicrobial agents.