Found programs: National Natural Science Foundation of China ( Nos . 81160447 , 81760878) ; Scientific Re- search Project of Jiangxi Provincial Department of Education (No . GJJ2200919) ; Jiangxi Provincial Key Laboratory of TCM Female Reproductive Health ( No . 20202BCD42015 ) ; Natural Science Foundation of Jiangxi Province (No . 20202BAB206073 , No . 20212BAB216066) ; Graduate Student Innovation and Entrepreneurship Training Project of Jiangxi Province (Grant No . S202210412069 , No . S202310412056) , Science and Technology Innova- tion Team of Jiangxi University of Chinese Medicine (No . CXTD22013)
Authors：Zhan Xiaoxuan1 , Liu Chengyi 1 , Peng Jiahua1 , Liu Shuzhen1 , Li Xin1 , Ren Yunying1 , Chen Danni 1 , Li Peishuang2 , Liang Ruining3
Keywords：Cinnamaldehyde; RPS7 ; endometriosis; proliferation; migration; invasion;apoptosis
DOI：10.19405/j.cnki.issn1000-1492.2025.03.004
〔Abstract〕 Abstract Objective To investigate the effects of cinnamaldehyde (CA) on the growth , metastasis and apoptosis of human endometriosis ( EMs) cells and to explore whether the mechanism is related to ribosomal protein S7 (RPS7) expression . Methods Endometriosis cells were divided into control group , CA group , sh-NC group , CA + sh-RPS7 group . Effects of CA on cell growth in human endometriotic cells were determined using Cell Counting Kit-8 (CCK-8) and colony formation assay . Effects of CA on cell metastasis were performed by motility assay and Transwell assay . Effects of CA on cell apoptosis were evaluated by Hoechst 33258 staining and flow cytometry . Meanwhile , the levels of PCNA , E-cadherin , Vimentin , Bax and Bcl-2 were evaluated using Western blot in hu- man endometriotic cells with treatment CA . The expression of RPS7 was detected by qRT-PCR and Western blot as- say . The RPS7 overexpression of human endometriotic cells was established by cell transfection . CA-mediated effects on cell proliferation and apoptosis were determined by CCK-8 assay and flow cytometry in human endometri- otic cells with RPS7 overexpression . Results CA repressed cell growth as well as down-regulated PCNA . The half inhibitory concentration ( IC50 ) value was 53 . 60 μmol/L after 24 h treatment , and colony formation rate was 25 . 32% . Additionally , CA inhibited metastasis which was associated with downregulated Vimentin and upregulated E-cadherin . The relative migration rates were 35% and 29% as well as invasion rate was 40% . Further , CA in- duced apoptosis by cell cycle G2/M phase arrest and cell apoptosis rate was 25 . 1% , which related to the up-regu- lation of of Bax and the down-regulation of Bcl-2 . CA inhibited the expression of RPS7 and overexpression of RPS7 promoted cell proliferation and suppressed apoptosis in CA-mediated 12Z cells . Conclusion CA inhibits cell growth , metastasis , and induces cell apoptosis by downregulating the expression of RPS7 .