〔Abstract〕 Abstract Objective To investigate the expressions of GPR15 and FOXP3 in colorectal carcinoma(CRC) tissues and their clinical prognostic values . Methods A total of 132 patients with CRC underwent radical surgery were collected . The control group selected the normal mucosal tissues more than 5 cm away from the edge of the cancer focus . Immunohistochemistry (Envision two-step method) was used to detect the expression levels of GPR15 and FOXP3 in CRC and adjacent tissues , and analyze their relationships with clinicopathological factors of colorectal cancer . Kaplan-Meier method was used to draw the survival curve to analyze the correlation between the expressions of GPR15 and FOXP3 and the survival prognosis of patients with CRC . The factors influencing prognosis of patients with colorectal cancer were analyzed by Cox regression . Results The immunohistochemistry showed that the ex- pression levels of GPR15 and FOXP3 in CRC were significantly higher than those in normal colorectal mucosal tis- sues (P < 0. 05) . The expression of GPR15 in CRC tissues was correlated with location , nerve invasion and TNM stage; FOXP3 expression was correlated with sex ( P < 0. 05) . Both expressions were not significantly correlated with the clinicopathologic features of age , tumor size , differentiation degree , tissue type , depth of invasion , tumor budding , vascular invasion and lymph node metastasis . Correlation analysis showed that there was no significant correlation between GPR15 and FOXP3 expression ( Kappa = - 0. 019 , P > 0. 05 ) . The survival prognosis of GPR15 positive group was significantly worse than that of negative group(log-rank:χ2 = 4. 3 , P = 0. 039) ;while the survival prognosis of FOXP3 positive group was significantly better than that of negative group(log-rank:χ2 = 7 . 3 , P = 0. 007) . Age ≤55 years , positive GPR15 and negative FOXP3 were independent risk factors for poor prognosis in patients with CRC(P < 0. 05) . Conclusion The expression levels of GPR15 and FOXP3 in CRC are signifi- cantly higher than those in paracancer tissues , GPR15 and FOXP3 are expected to become new tumor markers for early screening , accurate treatment and prognosis assessment of CRC .