〔Abstract〕 Objective To study the sensitivity of human cervical cancer cells to cisplatin which increased by the lactoferrin active hexapeptide(LfcinB 4-9) and to investigate its mechanism. Methods MTT was used to detect the proliferation inhibition of cisplatin(DDP) combined with different concentrations of LfcinB 4-9 on human cervical cancer Hela cell line and the Hela cell line with the p62 gene knocked out(Hela/KO p62). The plate cloning assay was performed to detect the effect of DDP combined with LfcinB 4-9 on the cloning ability of human cervical cancer cells. The effect of DDP combined with LfcinB 4-9 on the apoptosis of human cervical cancer cells was detected by flow cytometry. RT-qPCR and Western blot were used to detect the expression levels of genes and protein such as SIAH, PSMA, and β-catenin in cancer cells Hela and Hela/KO p62.Results The inhibitory rate of the combination of LfcinB 4-9 and DDP on cervical cancer cells was significantly higher than that of DDP alone or LfcinB 4-9 alone(P<0. 05 or P<0. 01). Compared with DDP group, the cloning ability of human ovarian cancer cells was significantly reduced, and apoptosis increased in combined treatment(P<0. 05 or P<0. 01). The qRTPCR and Western blot assay showed that when DDP combined with LfcinB 4-9, the expression of SIAH and PSMA1 increased, while the expression of β-catenin decreased.Conclusion The combined effect of LfcinB 4-9 and DDP significantly enhances the sensitivity of cervical cancer cells to DDP, which is achievedviathe proteasome pathway.