〔Abstract〕 Abstract Objective To investigate the relationship between the risk occurrence of rectal cancer and the single nucleotide polymorphisms (SNP) of zinc finger protein 6(ZBED6) , and to provide the experimental basis for early diagnosis and treatment of rectal cancer. Methods Polymerase chain reaction-restriction fragment length polymor- phism (PCR-RFLP) technology was used to genotype the ZBED6 gene in 109 randomly selected rectal cancer pa- tients and 110 unrelated healthy controls . To evaluate the relationship between alleles , genotypes and the risk of rectal cancer , unconditional Logistic regression analysis was used to OR and 95% CI. Results The SNP rs7552670 of ZBED6 had significant correlation with the risk of rectal cancer. Compared with the population carrying the TT genotype , those carrying the TC genotype had a 2. 653 fold increased risk of rectal cancer (TT vs TC : OR = 2. 635 , 95% CI = 1 . 501 - 4. 690) . Other SNPs had no significant correlation with the risk of rectal cancer. Conclusion There is an interaction between the polymorphisms of ZBED6(rs7552670) and rectal cancer. The population car- ried ZBED6 rs7552670 TC genotype had an growing risk of rectal cancer.