〔Abstract〕 To investigate the mechanism of action of curcumol on mitochondrial autophagy in hepatic stellate cells and its molecular mechanism against liver fibrosis. Methods Hepatic stellate cells were divided into blank group , model group ( lipopolysaccharide 5 mg/L) , and low , medium and high curcumol group ( 12. 5 , 25 and 50mg/L) ; Thiazolyland (MTT) was used to detect the effects of curcumol on the viability of hepatic stellate cells; flow cytometry was used to detect the effects of curcumol on apoptosis of hepatic stellate cells; 5 , 5 ′,6 , 6 ′- Tetrachloro-1 , 1 ′,3 , 3 ′-tetraethylimidacarbocyanine iodide ( JC-1) was used to detect the mitochondrial membrane potential; effects of curcumol on mitochondrial morphology and autophagosome were detected by transmission elec- tron microscopy; effect of curcumol on mitochondrial localisation were detected by fluorescent probe; Immunoblot- ting assay was performed to detect the effects of curcumin on PTEN-induced putative kinase 1 (PINK1) , Parkin- son ’s disease protein (Parkin) , microtubule-associated protein light chain 3 (LC3) , autophagy-associated protein (Beclin1) , mitochondrial inner membrane translocase 23 ( Timm23) , mitochondrial outer membrane translocase 20 ( Tomm20 ) , Bcl-2 associated X protein ( Bax) , B lymphocytoma-2 ( Bcl2 ) , cleaved-cysteine protease 3 (Caspase3) , α-smooth muscle actin ( ɑ-SMA) , collagen type Ⅰ (Collagen Ⅰ ) , and collagen type Ⅲ (Collagen Ⅲ) protein expression . Results Compared with the blank control group , cell proliferation rate , Caspase3 , Bcl2 , LC3 Ⅱ , Beclin1 , PINK1 , Parkin , ɑ-SMA , Collagen Ⅰ , CollagenⅢ proteins significantly increased in the model group (P < 0. 01) , co-localisation of mitochondria and lysosomes increased , and the number of mitochondrial auto- phagosome significantly increased (P < 0. 01) , while Timm23 and Tomm20 proteins , mitochondrial membrane po- tential decreased significantly (P < 0. 01) , apoptosis rate decreased , and Bax protein expression decreased . Com- pared with the model group , after curcumol intervention , cell proliferation rate , Bcl2 , Timm23 , Tomm20 , ɑ-SMA , Collagen Ⅰ and CollagenⅢ protein expression significantly decreased in the curcumol low - , medium - and high- concentration groups (P < 0. 01) , and the mitochondrial membrane potential significantly decreased (P < 0. 01) , whereas apoptosis rate , Caspase3 , Bax , LC3 Ⅱ , Beclin1 , PINK1 and Parkin proteins significantly increased (P < 0. 05) , the co-localisation of mitochondria and lysosomes increased , and the number of mitochondrial autophago- some significantly increased (P < 0. 01) . Conclusion Curcumol exerts ameliorative effects on hepatic fibrosis by modulating mitochondrial hyperautophagy mediated by the PINK1 /Parkin signaling pathway , and promoting hepatic stellate cell apoptosis .