Study of sodium distribution in rats with nephrotic syndrome

Acta Universitatis Medicinalis Anhui 2022 07 v.57 1054-1059     font:big middle small

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Authors:Ni Lianghou; Yang Mo; Li Manman; Wang Yunlai; Xuan Zihua; Xu Fan

Keywords:nephrotic syndrome;sodium retention;lymphatic;skin;sodium

DOI:10.19405/j.cnki.issn1000-1492.2022.07

〔Abstract〕 Objective To investigate the locations of sodium retention and the mechanism of its occurrence in the rat model of adriamycin nephrotic syndrome. Methods The nephrotic syndrome model was established by tail vein injections of adriamycin twice two weeks(4 mg/kg in first week and 2 mg/kg in second week). Urine and blood biochemical parameters were detected; the contents of water and sodium in rat skin were detected respectively by desiccation and atomic absorption spectrometry; the content of glycosaminoglycan was analyzed by ELISA; the expression of tonicity-responsive enhancer binding protein(TonEBP), vascular endothelial growth factor C(VEGFC), vascular endothelial growth factor receptor 3(VEGFR3) and lymphatic vessel hyaluronan receptor-1(LYVE-1) in the renal cortical was detected by Western blot; the lymphatic vessels distribution in the renal cortex was measured by immunofluorescence. Results the model rats had increased urinary protein excretion, and abnormal renal function and lipid, which suggested the model was successfully established. The excretion of Na+in urine decreased, the content of skin sodium and glycosaminoglycan in skin obviously increased, the expression of TonEBP, VEGFC, VEGFR3, LYVE-1 in renal cortical markedly increased, and density of lymphatic vessels in renal cortical notably increased(P<0.05 orP<0.01). Conclusion Sodium retained in nephrotic syndrome rats may be stored in the skin, which is related to the TonEBP/VEGFC/VEGFR3 pathway.