Found programs: Natural Science Foundation of Anhui Province (No . 2108085MH320) ; Research Project of An- hui Provincial Institute of Translational Medicine (No . 2023zhyx-C11)
Authors:Zong Xuemei 1 , Lin Qianqian1 , Chen Yuelan1 , Wang Xinming2 , Wei Wei 1 , Yan Shangxue1 , 3 , Chang Yan1 , 3
Keywords:rheumatoid arthritis; kynurenine 3-monooxygenase; kynurenine; 3-hydroxykynurenine; peripheral blood mononuclear cells; synovial tissue; fibroblast-like synoviocytes
DOI:10.19405/j.cnki.issn1000-1492.2025.07.008
〔Abstract〕 To investigate the expression and clinical significance of kynurenine-3-monooxygenase (KMO) in peripheral blood mononuclear cells ( PBMC ) , synovial tissue , and fibroblastic-like synovial cells (FLS) of rheumatoid arthritis (RA) patients . Methods Peripheral blood samples from 25 healthy control (HC) individuals and 25 patients diagnosed with RA were collected , and real-time fluorescence quantitative PCR and Western blot were used to detect KMO gene and protein expression in PBMC of RA and HC groups , and to analyze the correlation between the expression level of the KMO gene in the PBMC of the RA patients and the indexes of the laboratory tests . Meanwhile , immunohistochemistry and immunofluorescence were used to detect KMO expression in synovial tissue and FLS in RA and HC groups . Results ① KMO gene and protein expression in PBMC of RA group were higher than that of HC group , and the difference was statistically significant (P < 0. 001) . ② The level of KMO gene expression in PBMC of RA group was positively correlated with disease activity index 28 score , blood sedimentation , and rheumatoid factor ( rs = 0. 417 , P = 0. 038 ; r = 0. 545 , P = 0. 005 ; rs = 0. 433 , P = 0. 031) , and had no correlation with C-reactive protein and anti-cyclic citrullinated polypeptide antibody . ③ KMO expres- sion in synovial tissue of RA group was higher than that of HC group , and the difference was statistically significant (P < 0. 01) ; KMO expression in FLS of synovial tissue of RA group was higher than that of HC group , and the difference was statistically significant ( P < 0. 001) . Conclusion KMO expression increases in PBMC , synovial tissue and FLS of RA patients , and the level of KMO gene expression is correlated with the disease activity of RA patients , suggesting that KMO may promote the course of RA .