Found programs: National Natural Science Foundation of China(No.42177416);Medical Research Key Project of Hubei University of Science and Technology(No.2022YKY01);Project in Science and Technology Planning of Xianning City(No.2023SFYF095);Scientific Research Innovation Team Project of Hubei University of Science and Technology(No.2023T08)
Authors：Huang Qi; Zhu Deyu; Liang Xiao; Wu Jinling; Qin Wengui; Ma Ping; Wu Yang; Bao Cuiyu
Keywords：polystyrene microplastics; oxidative stress; ferroptosis; myocardial damage; environmental contaminants;
DOI：10.19405/j.cnki.issn1000-1492.2025.06.005
〔Abstract〕 Objective To investigate the effect of polystyrene microplastics(PS-MPs) on myocardial injury in mice and its molecular mechanism. Methods A total of 60 male C57BL/6 mice were randomly divided into normal saline group, 0.1, 1, 10 mg/kg PS-MPs exposed group, and doxorubicin [5 mg/(kg·w)] group treated for 8 weeks. After treatment, we measured blood pressure, cardiac organ coefficient, cardiac histopathological changes, oxidative stress markers reactive oxygen species(ROS), malondialdehyde(MDA), glutathione(GSH), 4-hydroxynonenal(4-HNE) and nuclear factor E2-related factor 2(Nrf2), serum centroid injury markers creatine kinase MB(CK-MB) and troponin(cTnT), ferroptosis marker recombinant glutathione peroxidase 4(GPX4), Recombinant solute carrier family 7(SLC7A11) as well as ferrous ions(Fe2+). Results Compared with the negative control group, vacuolation, inflammatory infiltration and collagen fiber deposition were evident in the hearts of mice after PS exposure. The levels of myocardial injury markers CK-MB and cTnT significantly increased. Cardiac organ coefficient decreased, blood pressure increased, oxidative stress markers and ferroptosis markers increased. Conclusion PS-MPs exposure can induce oxidative stress and activate ferroptosis pathway, resulting in myocardial injury in mice.