〔Abstract〕 Objective To investigate the hemolytic phenotypes of Staphylococcus aureus clinical isolates. Methods The hemolytic phenotypes of 105 Staphylococcus aureus isolates were analyzed and summarized using the three-point inoculation method. Real-time fluorescence quantitative PCR was used to measure the mRNA expression levels of four hemolysin genes(hla, hlb, hlc, and hld); The VITEK 2 GP639 antimicrobial susceptibility card was used to detect resistance to commonly used antibiotics; DNA gel electrophoresis was performed to determine the prevalence of the mecA,sea,tst,and pvl genes; The microtiter plate crystal violet staining method was used to assess biofilm formation ability; The CCK-8 assay was used to evaluate cytotoxicity against macrophages. Results Seven hemolytic phenotypes were identified among the Staphylococcus aureus clinical isolates. Differences were found among Staphylococcus aureus clinical isolates with different hemolytic phenotypes in terms of mRNA expression levels of hemolysin genes,antibiotic resistance,virulence gene prevalence,biofilm formation ability,and cytotoxicity to mouse macrophages(P