〔Abstract〕 Objective The main purpose of this study is to explore the relationship between hepafin-binding epidermal growth factor-like growth factor(HB-EGF) and urokinase-type plasminogen activator receptor(uPAR) with the pathogenesis of preeclampsia. Methods Firstly, the differentially expressed proteins were screened by antibody chip technology, a large number of literatures were searched, and uPAR and HB-EGF were selected for verification. Serum samples from 30 normal pregnant women and 50 preeclampsia pregnant women were collected, and the expressions of HB-EGF and uPAR in serum were determined by ELISA. Placental tissues of 10 preeclampsia pregnant women and 6 normal pregnant women were selected for further validation by qRT-PCR and immunohistochemistry. Results The expression of u PAR in serum in preeclampsia pregnant women was higher than that in normal pregnant women( P<0. 05),and the average level of HB-EGF in serum was lower than that of normal pregnant women( P<0. 05). The results of qRT-PCR showed that the expression level of u PAR mRNA in placenta was low or no,and the expression level of HB-EGF mRNA in preeclampsia pregnant was lower than that in normal pregnant women( P<0. 05). Immunohistochemistry showed that the expression of u PAR and HB-EGF in the placenta of preeclampsia pregnant was lower than that of normal pregnant women. Conclusion The abnormal expression of HBEGF and u PAR is related to preeclampsia. This study proves that HB-EGF and u PAR are related to the pathogenesis of preeclampsia,and the difference in the expression of HB-EGF and u PAR in blood may play a guiding role in the early diagnosis of biomarkers.