〔Abstract〕 Objective To analyze the changes of intestinal flora and explore the possible mechanisms of alcoholic liver injury in mice. Methods Six-week-old mice were randomly divided into control group(Ctrl group) and alcohol group(EtOH group). The EtOH group was fed with Lieber-DeCarli liquid feed for 11 days and then given a single alcohol gavage to establish a chronic and acute alcoholic liver injury model. The Ctrl group was fed with liquid control diet and given isocaloric dextrin by gavage. Serum, liver, intestinal tissue and fecal samples were collected 9 h after gavage. The levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and triglyceride(TG) in serum and hepatic tumor necrosis factor(TNF)-α, interleukin(IL)-6, IL-8, IL-1β mRNAs were measured. The pathological changes of liver and intestine were observed by HE staining. Illumina highthroughput sequencing was used to detect α diversity, β diversity and species composition at phylum and genus levels.Results Compared with the Ctrl group, alcohol exposure significantly increased serum ALT and AST in mice(P<0.01), and obviously increased liver IL-6, IL-8 and IL-1β mRNA(P<0.01). HE staining showed that there were scattered cell necrosis and inflammatory infiltration in the liver of mice in EtOH group. The intestinal structure was not clear, the gland was atrophy, and there was obvious inflammatory infiltration. The structure of intestinal flora in EtOH group changed,Firmicutesabundance increased at phylum level(P<0.05),Parasutterella abundance decreased at genus level(P<0.05), andClostridiaceaeandClostridium-sensu-strictowere the key genera.Conclusion The composition of intestinal microflora in mice with alcoholic liver injury changes, which may be related to the occurrence of liver disease.