Found programs: National Natural Science Foundation of China ( No . 82360588 ) ; National Natural Science Foundation Incubation Plan of the Affiliated Hospital of Guizhou Medical University ( No . gyfynsfc[2023]-17 ) ; Scientific and Technological Project of Guizhou Province (No . Qian Kehe Basic-ZK[2024] General 202) ; Scien- tific and Technological Project of Guizhou Health Commission (No . gzwkj2024-204)
Authors：Zhang Yu1 , 2 , Zhu Xiaoyu1 , 2 , Xu Dianqin1 , 2 , Chen Xiaowei2 , Zhong Mingyan2 , Zhou Xinzhu1 , 2 , Tan Yujie1 , 2
Keywords：cervical cancer;COL1A2;tumor immunity;PAX5;immune infiltration
DOI：10.19405/j.cnki.issn1000-1492.2025.10.005
〔Abstract〕 To explore the expression of collagen type 1 alpha 2 (COL1A2) in cervical cancer and its correlation with immune infiltration . Methods Bioinformatics techniques were used to analyze the expression of COL1A2 in cervical cancer. Western blot and RT-qPCR were used to detect the expression of COL1A2 in cervical cancer tissues and cell lines . The correlation between the expression of COL1A2 and tumor immune cell infiltration was analyzed by tumor immune estimation resource (TIMER2 . 0) . Gene set enrichment analysis (GSEA) was used to analyze the possible mechanism of COL1A2 in cervical cancer. Jaspar database was used to predict the transcrip- tion factors of COL1A2 . Western blot and RT-qPCR were used to detect the expression of transcription factors in cervical cancer tissues and cell lines . Results The expression of COL1A2 was down-regulated in cervical cancer (P < 0. 05) . The expression of COL1A2 was positively correlated with the levels of macrophages and myeloid den- dritic cells (P < 0. 01) . The proportions of 22 types of immune cells were different in different cervical cancer pa- tients . In addition , compared with the high expression group of COL1A2 , the proportion of M0 macrophages , M2 macrophages and resting memory CD4 + T cells increased in the low expression group of COL1A2 , while the propor- tion of CD8 + T cells , activated memory CD4 + T cells , follicular helper T cells , activated NK cells and activated myeloid dendritic cells decreased (P < 0. 05) . GSEA analysis showed that COL1A2 was related to immune-related signaling pathways , including Notch signaling pathway , interleukin-6/janus kinase/signal transducer and activator of transcription 3 (IL6/JAK/STAT3) , Wnt/β-catenin signaling pathway , etc . (P < 0. 01) . Jaspar database pre- dicted that the transcription factor of COL1A2 was paired box protein 5 (PAX5) , and the expression of PAX5 de- creased in cervical cancer (P < 0. 05) . Conclusion COL1A2 is expected to become a potential diagnostic biomar- ker and immunotherapy target for cervical cancer.