Found programs: Natural Science Foundation of Inner Mongolia Autonomous Region (No . 2024MS08079) ; Inno- vation Team Development Plan Project of Baotou Medical College (No . bycxtd-06) ; Scientific Research Fund Pro- ject of Baotou Medical College (No . BYJJ-GDJY 202313)
Authors：Chu Ning1 , 2 , Dong Wenjie1 , Gao Fang3 , Li Yingze4 , Chen Yaru1 , Zhang Bin5 , Jia Yanbin1
Keywords：non-cardia gastric cancer; Helicobacter pylori; arachidonate15-lipoxygenase; single nucleotide poly- morphism; genetic susceptibility; association study
DOI：10.19405/j.cnki.issn1000-1492.2025.10.012
〔Abstract〕 To explore the association between single nucleotide polymorphism (SNP) in the arachido- nate 15-lipoxygenase (ALOX15) gene and Helicobacter pylori (H. pylori) infection as well as the risk of non-cardi- a gastric cancer in Baotou Han population , and to provide experimental evidence and data support for the screening of susceptible population for non-cardia gastric cancer. Methods A total of 458 cases with non-cardia gastric canc- er and 460 healthy examination people were collected . The 14 C urea breath test (UBT) and enzyme-linked immu- nosorbent assay (ELISA) were used to detect H. pylori infection in the 460 healthy individuals . The genotypes of ALOX15 rs2619112 , rs2619118 , rs2664593 , rs7220870 were detected by polymerase chain reaction-restriction fragment length polymorphism , and the association of SNP with H. pylori infection as well as the risk of non-cardia gastric cancer was statistically analyzed . Results The positive rate of H. pylori infection was 42. 4% . ALOX15 rs2619112 , rs2619118 , rs2664593 , and rs7220870 had no association with H. pylori infection . ALOX15 rs2619112 , rs2664593 , and rs7220870 were not associated with the risk of non-cardia gastric cancer. Compared with the carriers of (CC + CT) genotype , the carriers of rs2619118 TT genotype had an increased onset risk of non-cardia gastric cancer [ OR (95% CI) = 1 . 512 ( 1 . 110 - 2. 060 ) ] . The haplotype ACCC constructed by ALOX15 rs2619112 , rs2619118 , rs2664593 , and rs7220870 could reduce the onset risk of non-cardia gastric cancer . The second-order interaction of ALOX15 rs2619112 and rs2619118 was associated with the risk of non-car- dia gastric cancer ( P < 0. 05) . Conclusion ALOX15 rs2619112 , rs2619118 , rs2664593 , rs7220870 may not play a major role in H. pylori infection . ALOX15 rs2619118 TT genotype is a risk factor for the development of non-cardia gastric cancer. The haplotype ACCC constructed by ALOX15 rs2619112 , rs2619118 , rs2664593 , and rs7220870 reduces the onset risk of non-cardia gastric cancer. The interaction of ALOX15 rs2619112 and rs2619118 has a synergistic effect in the development of non-cardia gastric cancer.