Found programs: National Natural Science Foundation of China ( No . 82360588 ) ; National Natural Science
Authors：Zhang Yu1 , 2 , Zhu Xiaoyu1 , 2 , Xu Dianqin1 , 2 , Chen Xiaowei2 , Zhong Mingyan2 , Zhou Xinzhu1 , 2 , Tan Yujie1 , 2
Keywords：cervical cancer; COL1A2; tumor immunity; PAX5; immune infiltration;
DOI：10.19405/j.cnki.issn1000-1492.2025.10.005
〔Abstract〕 Objective To explore the expression of collagen type 1 alpha 2(COL1A2) in cervical cancer and its correlation with immune infiltration. Methods Bioinformatics techniques were used to analyze the expression of COL1A2 in cervical cancer. Western blot and RT-qPCR were used to detect the expression of COL1A2 in cervical cancer tissues and cell lines. The correlation between the expression of COL1A2 and tumor immune cell infiltration was analyzed by tumor immune estimation resource(TIMER2.0). Gene set enrichment analysis(GSEA) was used to analyze the possible mechanism of COL1A2 in cervical cancer. Jaspar database was used to predict the transcription factors of COL1A2. Western blot and RT-qPCR were used to detect the expression of transcription factors in cervical cancer tissues and cell lines. Results The expression of COL1A2 was down-regulated in cervical cancer(P+ T cells increased in the low expression group of COL1A2, while the proportion of CD8+ T cells, activated memory CD4+ T cells, follicular helper T cells, activated NK cells and activated myeloid dendritic cells decreased(P