Found programs: Natural Science Foundation of Hebei Provincial ( No . H2021406056) ; Scientific and Techno- logical Research Project for Higher Education Institutions in Hebei Province ( No . ZD2020178) ; College Student Innovation and Entrepreneurship Training Project in Chengde Medical University (Nos . 2022014 , 2024074)
Authors：Zheng Mengyuan1 , 2 , Hao Zitong1 , 2 , Zhu Qinghua1 , 2 , Tian Zhuangzhuang1 , Guo Xingda1 , Zheng Yuhe1 , Li Cheng1 , Fu Xiumei 1 , 3
Keywords：acellular nerve allografts; sciatic nerve injury; Bcl-2/Cyt-C/Apaf-1 signalling pathway; spinal cord; motor neurons; rats;
DOI：10.19405/j.cnki.issn1000-1492.2025.11.007
〔Abstract〕 Objective To investigate the protective effects and mechanisms of acellular nerve allografts(ANA) on motor neurons in the spinal cord anterior horn of sciatic nerve injury(SNI) rats. Methods SPF grade male SD rats were randomly divided into normal, model, ANA-bridged(bridge group), and autologous nerve transplantation groups(autograft group), with 6 rats in each group. The SNI rat model was established using the right sciatic nerve clamp method for 10 mm. In the bridge group, the ANA was bridged to the two severed ends of the injured sciatic nerve, and in the autograft group, the autologous nerves were flipped head to tail and then bridged to the two severed ends. A spectrophotometer was applied to determine the DNA content in normal nerves and ANA. The footprint test was used to determine the sciatic nerve function index(SFI) of the rats in each group, the wet weight ratio of the anterior tibialis muscle was calculated. The morphology and structure of the anterior horn motor neurons of the spinal cord of each group were observed by HE staining. The immunofluorescence and Western blot were used to detect Apaf-1, Caspase-3, Bcl-2, Bax, and Cyt-C proteins expression in the L4-6 segment of the spinal cord. Results The DNA content in the ANA prepared in this study was significantly lower than that in normal nerves(P