〔Abstract〕 To explore the microbiological characteristics of Staphylococcus epidermidis with hemolytic phenotype（SEHP）. Methods Hemolytic phenotype was detected using the three-point inoculation method，in- volving a total of 5 strains of SEHP and 5 strains of Staphylococcus epidermidis with non-hemolytic phenotype （SENHP）. Bacterial species were identified using the Microflex LT MALDI-TOF mass spectrometer，and a phylo- genetic tree was constructed through 16S rRNA sequence alignment. Growth curves were monitored through the mi- crocultivation assay. Biofilm formation ability was assessed by microplate crystal violet staining. Red blood cell tox- icity was detected using the microplate method. Antimicrobial susceptibility testing of SEHP and SENHP against commonly used antibiotics was performed using a VITEK 2 GP639 test kit. Antagonistic effects of SEHP and SENHP against Staphylococcus aureus and Corynebacterium striatum were evaluated by the Oxford cup inhibition as- say. Results Compared with SENHP，SEHP exhibited a marked decrease in growth rate during the late logarithmic phase，accompanied by significant hemolytic toxicity. Additionally，it showed lower resistance rates to levofloxacin and moxifloxacin，and could antagonize Staphylococcus aureus and Corynebacterium striatum. Conclusion The mi- crobiological characteristics of SEHP differ from those of SENHP in that SEHP demonstrates antagonistic effects against S. aureus and C. striatum.