〔Abstract〕 Objective To explore the role of rat Sesn2/AMPK signal-mediated liver autophagy in the abnormal glucose metabolism caused by intermittent hypoxia and reoxygenation. Methods 48 rats were randomly divided into a control group(NC) and chronic intermittent hypoxia(CIH) group, with 24 rats in each group.The CIH group was placed in an intermittent hypoxia chamber for 8 h/d for 8 weeks, and then fed under normoxia for 4 weeks; The NC group was always fed under normoxia.The levels of fasting blood glucose(FBG),fasting insulin(FINS),liver Sesn2,AMPK,p-AMPK protein, mTOR and LC3 mRNA levels were measured at baseline, 8 weeks of intermittent hypoxia, and 4 weeks of reoxygenation in the two groups of rats. Results At baseline, there was no statistically significant difference in the above indicators between the two groups(P>0.05); at 8 weeks of intermittent hypoxia, compared with the NC group, the levels of FBG,FINS,HOMA-IR and mTOR in the CIH group increased, the levels of Sesn2,p-AMPK/AMPK and LC3 decreased, and the difference was statistically significant(P<0.05); after 4 weeks of reoxygenation, there was no statistically significant difference in the two groups′ indexes(P>0.05). Conclusion Chronic intermittent hypoxia can cause the increase of FBG and FINS in rats, which may be caused by inhibiting Sesn2/AMPK-mediated liver autophagy and causing insulin resistance.