〔Abstract〕 Objective To confirm the therapeutic angiogenesis of danhong injection(DHI) in acute myocardial infarction(AMI) of rats.Methods Forty rats were randomly divided into five groups: Sham group, Model group,DHI low-dose(0. 5 ml/kg) group, mid-dose(1. 0 ml/kg) group, high-dose(2. 0 ml/kg) group. The mouse model of AMI was induced by ligation of the left anterior descending coronary artery. Rats were treated with different doses of DHI(0. 5, 1. 0, 2. 0 ml/kg) for 7 days. Cardiac function of rats was indicated by left ventricular ejection fraction(LVEF) and fractional shortening(LVFS), serum levels of troponin T(TN-T) and brain natriuretic peptide(BNP), myocardial pathological damage, CD34 positive microvessel density(MVD), the expression of Von willebr and factor( vWF), alpha-smooth muscle actin （ α-SMA） and vascular endothelial growth factor-A(VEGF-A) were measured by echocardiography, enzyme linked immunosorbent assay, HE staining, immunohistochemistry and Western blot, respectively.Results Compared with Sham group, LVEF,LVFS in Model group increased(P<0. 01), serum levels of TN-T and BNP decreased(P<0. 01), α-SMA(P<0. 05), CD34,vWF,VEGF-A(P<0. 01) were up-regulated. Compared with Model group, LVEF,LVFS and the expression of CD34,vWF in mid-dose and high-dose groups increased(P<0. 05,P<0. 01). TN-T,BNP in all dosage groups were reduced while the expression of α-SMA,VEGF-A were further upgraded(P<0. 05,P<0. 01). Besides, the pathological damage in the marginal zone of infarcted myocardium was lessened.Conclusion DHI has an effect on therapeutic angiogenesis for AMI in rats.