〔Abstract〕 Objective To analyze the clinical data, biochemical indexes and distribution characteristics of patients with embryonic chromosome abnormalities with early spontaneous abortion, and to explore the risk factors that may cause embryonic chromosomal abnormalities. Methods 315 patients with early spontaneous abortion and chromosome detection of abortion tissue were selected.According to the result of the chromosomes of villi tissue microarray analysis(CMA),315 patients were divided into abnormal group(165 cases) and normal group(150 cases).The clinical data and biochemical indexes of the two groups were analyzed and compared.The screened variables with statistically significant differences were analyzed by multiple Logistic regression. Results The rate of chromosome abnormality in early pregnancy spontaneous abortion embryos was 52.38%.Aneuploid, polyploid, and chromosome structural abnormalities accounted for 71.52%,8.48% and 20.00%,respectively.Compared the abnormal group with the normal group, the distribution of the male age, female nationality, education, career, life history, birth history and previous medical history had no statistically significant differences(P>0.05).There were no statistically significant differences between the two groups in the levels of body mass index(BMI),serum antinuclear antibodies(ANA),anticardiolipin antibody(ACA),antithyroid peroxidase antibody(TPOAb),antithyroglobulin antibody(TgAb),fasting insulin(FINS),fasting blood sugar(FPG),homocysteine(HCY),and sex hormone(P>0.05).Compared the abnormal group with the normal group, the differences of female age, method of conception, serum 25-(OH)D and thyroid stimulating hormone(TSH) had statistical significance(P<0.05).Multivariate analysis showed that female advanced age and vitamin D deficiency were risk factors for fetal chromosomal abnormalities. Conclusion Embryonic chromosome abnormalities are related to female age, method of conception, levels of serum 25-(OH)D and TSH.Female advanced age and vitamin D deficiency may increase the risk of embryonic chromosome abnormalities.