〔Abstract〕 Objective To observe the effect of mTOR pathway on glycolysis of T cells after Echinococcus granulosus infection. Methods Balb/c mice were randomly divided into control group, infection group and inhibitor group. In the infection group and inhibitor group, protoscolex was injected under the liver capsule, the control group was injected with an equal volume of PBS, and the inhibitor group was intraperitoneally injected with rapamycin(1.25 mg/kg) daily from the next day. At 15, 30, and 70 days after infection, peripheral blood and spleen were collected, qRT-PCR was used to detect the mRNA expression of metabolic enzymes, and Western blot was used to detect the protein expression of the mTOR signaling pathway. Results Compared with the control group, the protein expression of HIF-1α, P-p70 S6 and P-4 EBP-1 downstream of the mTOR pathway in the infected group decreased(P<0.05), and the mRNA expression of glycolysis-related transcription factors HK3, PKM2, LDHA and MCT4 decreased(P<0.05). After rapamycin inhibited the mTOR pathway, the mRNA expression of glycolysis-related transcription factors HK3, PKM2, LDHA and MCT4 were further reduced(P<0.001). Conclusion Echinococcus infection inhibits glycolytic activity in T cells through the mTOR pathway.