〔Abstract〕 Peripheral blood of healthy people was used to induce the proliferation and differentiation of B lymphocytes into plasmablasts and plasma cells in vitro.Cells characteristics were observed under microscope.Molecules expression level of B cells surface was analyzed under flow cytometry.Cell viability was observed by Trypan blue.The result showed CD40 L signal stimulation, CpG immunoregulatory sequence combination and optimized cytokine combination could effectively promote the transformation of human B lymphocytes and the establishment of vitro model.