〔Abstract〕 Objective To explore the role and molecular mechanism ofSupervillinin zebrafish embryo development. Methods Clustal Omega and DNAman software were used to analyze and compare the homology of amino acid sequences of Svil and SVIL in zebrafish and human. The expression pattern ofSvila(subtype of Svil protein in zebrafish) during early embryonic development was analyzed by RT-PCR and in situ hybridization.Svilaexpression in zebrafish was inhibited by injecting morphinos(MO), and morphological changes of embryos were observed. The expression and nuclear localization of β-catenin protein were detected by Western blot, and the expression ofWnt/β-catenintarget gene was detected by RT-PCR. Results During the early embryonic development of zebrafish,Svilawas maternally expressed and showed an upward trend with the development process. The expression ofSvilawas reduced by MO, and the development of zebrafish embryos was distorted and the body axis was bent, which might be related to the blocked movement of concentrated extension of embryos. Further studies showed thatSvilaexpression affectedβ-cateninnuclear transport andWnt/β-cateninsignaling pathway activation. Conclusion Svilaregulates the concentrated extension movement of zebrafish embryos by activating theWnt/β-cateninsignaling pathway.