〔Abstract〕 Objective To analyze and compare the gene chip data of normal people and patients with acute myocardial infarction through GEO gene expression database, to screen out differentially expressed genes(DEGs), and to predict potential Chinese medicines for the treatment of acute myocardial infarction. Methods GSE66360 gene microarray was downloaded, DEGs information was obtained by analysis, gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis of differential genes were performed, key genes were further analyzed by String database and Cytoscape software, and key genes were mapped to the medical ontology information retrieval platform(Coremine Medical) to screen potential Chinese medicines for the treatment of acute myocardial infarction. Results A total of 943 differentially expressed genes were screened. The biological process was mainly enriched in myeloid leukocyte activation, regulation of cytokine production, leukocyte chemotaxis, etc. The cellular component was mainly focused on secretory granule lumen, membrane surface, and extrinsic components of the membrane, etc. Molecular function was mainly in chemokine receptor binding, pattern recognition receptor activity, cytokine binding, etc. KEGG analysis showed that the main signaling pathways involved were tumor necrosis factor(TNF), hypoxia inducible factor-1(HIF-1), and JAK-STAT signaling pathways, etc. The key genes to be screened are formyl peptide receptor 2(FPR2), signal transducer and activator of transcription 3(STAT3), chemokine(C-X-C motif) ligand 1(CXCL1), chemokine(C-X-C motif) ligand 8(CXCL8), ubiquitin protein ligase E3 component n-recognin 4(UBR4), jun proto-oncogene(JUN), platelet-activating factor receptor(PTAFR), Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide(FCER1 G), G protein-coupled receptor 84(GPR84), plasminogen activator, urokinase(PLAU). The potential herbs predicted for the treatment of acute myocardial infarction were Centipede(P=0.003 30), Rithoma Curcuma(P=0.002 39), Curcuma(P=0.002 40), Paris polyphylla Smith(P=0.002 48), Salviae miltiorrhizae(P=0.002 72), Fritillary bulb(P=0.003 71), and Panax ginseng(P=0.001 59). Conclusion Traditional Chinese medicines such as Rithoma curcuma in activating blood and removing blood stasis medicine, Panax ginseng in nourishing Qi, and Fritillary bulb in medicine for the treatment of cough and asthma have protective effects on acute myocardial infarction. The mechanism of action may be related to the regulation of immune and anti-inflammatory signaling pathways.