〔Abstract〕 Objective To explore the effect of knocking down WD repeat domain 12(WDR12) on mouse spermatogonial cells(GC-1). Methods After transfecting cells with shWDR12 and negative control EGFP plasmids by liposome transfection, Western blot and Q-PCR were used to detect the expression of WDR12 in the cells; CCK-8 kit detected cell proliferation; TUNEL kit stains detected cell apoptosis; Q-PCR detected the differential expression of ribosomal protein genes in cells. Results Compared with the cells transfected with the negative control plasmid, the detection results of the cells transfected with shWDR12 showed that the expression of WDR12 in GC-1 cells was successfully reduced(P<0.01); the proliferation of GC-1 cells knocked down by WDR12 was inhibited; the results of TUNEL staining showed that the number of apoptotic cells increased after WDR12 knockdown; the expression of ribosomal protein genes decreased after shWDR12 transfection, ribosomal protein S3(RPS3)(P<0.05), ribosomal protein L11(RPL11)(P<0.01), ribosomal protein S15(RPS15)(P<0.05), and ribosomal protein L31(RPL31)(P>0.05). Conclusion Transfection of shWDR12 can effectively knock down the expression of WDR12 in GC-1 cells. The decrease of WDR12 expression leads to a decrease in the proliferation ability of GC-1 cells, an increase of apoptosis and the down-regulation of ribosomal-related protein gene expression.