〔Abstract〕 Objective By testing the tensile strength of sodium alginate bacterial cellulose composite membrane loaded with nano silver, the antibacterial property ofStaphylococcus aureusand its effect on the proliferation of mouse embryonic fibroblasts were studied to explore its feasibility as a new type of guided bone tissue regeneration membrane. Methods The sodium salt(SA) solution was uniformly mixed with bacterial cellulose(BC) solution in proportion(the mass fraction ratio between SA and BC was 10 ∶3, 10 ∶5, 10 ∶7, 10 ∶9 and pure SA membrane), then the membrane was self-evaporating and the silver nanoparticles(AgNPs) were produced by in-situ reduction of dopamine on the membrane surface. The tensile strength of each film specimen was tested by universal mechanical testing machine.The microstructure of composite film with the maximum tensile strength was observed by scanning electron microscope and the composition was analyzed by X-ray diffractometer. The biocompatibility of the composite membrane was tested by cell counting kit. The antibacterial activity was tested by inhibition zone method. Results Silver nanoparticles were uniformly distributed in the Ag-SA/BC composite film, and X-ray diffraction analysis showed that AgNPs, sodium alginate and BC existed in the composite film. When the mass fraction ratio of SA to BC was 10 ∶7, the tensile strength of the composite membrane reached(223.8±9.9) MPa. The results of cell experiments showed that the AG-SA/BC composite membrane had excellent biocompatibility.Staphylococcus aureusshowed clear inhibition zone after incubation for 24 h. Conclusion When the Ag-SA/BC mass fraction ratio was 10 ∶7, the tensile strength of the composite membrane is the highest, which shows excellent antibacterial property toStaphylococcus aureusand good cellular compatibility.