〔Abstract〕 Objective To study the drug-resistant characteristics of group BStreptococcusin urinary tract infection, the distribution of drug resistance genes and sensitivity of CAMP test and thecfbgene expression. Methods The sensitivity of antimicrobial agents was determined by disk diffusion method and Vitek 2 Compact instrument analysis. The erythromycin induced clindamine resistance was detected by D test. The antibiotic resistance genes were detected by PCR. CAMP test was conducted and strains were identified by detecting 16 S rRNA gene fragments and thecfbgene, biochemical test and MALDI-TOF MS. Results The drug resistance rates of 51 strains of group BStreptococcusto ampicillin, doxycycline, rina thiazole amine, nitrofurantoin, penicillin and quinupristin/dalfopristin, tigecycline, vancomycin were 0, while the drug resistance rates to erythromycin, tetracycline, clindamycin, levofloxacin, moxifloxacin, and ciprofloxacin were 66.7%, 66.7%, 49.0%, 47.1%, 49.0%, and 47.1%, respectively. The main erythromycin and tetracycline resistance genes were ermB and tetM respectively, and quinolones resistance genes were gyrA and parC and their detection rate was all 78.4%. The results of CAMP test showed that 3 strains were negative, 9 strains were weakly positive and 39 strains were positive. Combined with biochemical tests,cfbgene, 16 S rRNA detection and MALDI-TOF MS identification results, all 51 strains were group BStreptococcus. However,cfbgenes of 2 CAMP-negative strains and 2 weakly positive strains were not detected. Conclusion Group BStreptococcusin urinary tract infection is sensitive to a variety of antibiotics, but highly resistant to erythromycin, clindamycin, tetracycline and quinolone antibiotics. There is a certain correlation between drug-resistant gene and drug resistance. CAMP test andcfbgene expression are not sensitive to the identification of group BStreptococcus.