〔Abstract〕 Objective To explore the associations between rs11225163 and rs1820453 of Yes associated protein(YAP) gene and the risk of Helicobacter pylori(H.pylori) infection and non-cardia gastric cancer, and to provide new ideas for the prevention and treatment of gastric cancer and diseases related to H.pylori infection. Methods Taqman method was used to genotype 427 cases with non-cardia gastric cancer and 381 controls.Enzyme linked immunosorbent assay(ELISA) was used to detect the titer of the anti-H.pylori antibody in the peripheral blood of normal people.The Unconditional Logistic regression method was used to calculate the odds ratio(OR) and 95% confidence interval(CI) to evaluate the associations betweenYAPSNPs and the infection of H.pylori and non-cardia gastric cancer under Codominant, Dominant, Overdominant, Recessive, and Log-additive genetic models.Haploview 4.2 software was used to construct haplotypes. Results No associations were found between SNP rs11225163 and rs1820453 and infection of H.pylori under Codominant, Dominant, Overdominant, Recessive, and Log-additive genetic models.No haplotype was constructed between the two sites.No associations were found between SNP rs11225163 and rs1820453 and the risk of non-cardia gastric cancer under Codominant, Dominant, Overdominant, Recessive, and Log-additive genetic models.There was no haplotype between the two sites. Conclusion YAPrs11225163 and rs1820453 may not play a major role in H.pylori infection and the risk of non-cardia gastric cancer.