〔Abstract〕 Objective To screen molecular markers of nasopharyngeal carcinoma(NPC) by establishing a microRNA(miRNA) regulatory network. Methods The miRNA chip GSE70970 and gene chip GSE12452 of NPC were downloaded from Gene Expression Omnibus(GEO) database. The differentially expressed miRNAs(DEMs) and differentially expressed genes(DEGs) were screened by R software and functional enrichment was analyzed. The transcription factors and target genes of DEMs were predicted by FunRich software. The intersection of target genes and DEGs was defined as hub gene, and the miRNA-mRNA regulatory network was established by Cytoscape software. The survival analysis of hub genes was performed by the Kaplan-Meier plotter database. Results A total of 47 DEMs and 797 DEGs were screened. Functional analysis showed that DEMs were found to be associated with signal transduction and transcription regulation, DEGs were mainly involved in cytokine cytokine receptor interaction, cell cycle, small cell lung cancer and other signaling pathways. 23 hub genes were obtained and the miRNA-mRNA regulatory network was established. The survival analysis showed that the expression level of 4 hub genes were associated with prognosis of NPC patients, including multiple inositol-polyphosphate phosphatase 1(MINPP1),sentan(SNTN), zinc and ring finger 3(ZNRF3) and tubulin beta 2 A(TUBB2 A). The corresponding miRNAs were hsa-miR-199 b-5 p,hsa-miR-520 e,hsa-miR-107,hsa-miR-421. Conclusion By establishing miR NA-mRNA regulatory network and survival analysis,molecular markers of NPC are screened to provide potential targets for diagnosis and treatment of NPC.