〔Abstract〕 Objective To research the effects of different concentrations of galangin( Ga) on the proliferation and migration of the A549 and PC9 cells of human lung adenocarcinoma and their possible mechanisms. Methods A549 and PC9 cells were cultured in vitro and were divided into control group( DMSO solvent control group,0 Ga control group) and experimental groups, which contained different concentrations( 12. 5,25,50 and 100μmol/L),respectively. CCK-8 test and cell scratch test were used to detect the effects of the proliferation and migration of lung adenocarcinoma cells in each group. The changes of lactic acid( LD) production,lactate dehydrogenase( LDH) activity and ATP production were detected by biochemical tests. The expression of E-cadherin( Ecad) and N-cadherin( N-cad) in cells was detected by ELISA kits. Differences between groups in each index were compared by statistical analysis. Results CCK-8 results showed that Ga had an inhibitory effect on the proliferation of lung adenocarcinoma cells. Cell scratch results showed that Ga had an inhibitory effect on the migration of lung adenocarcinoma cells. Biochemical detection results showed that Ga had an inhibitory effect on LD production,LDH activity and ATP production. ELISA results showed that E-cad protein expression in the supernatant in the experimental groups was higher than that in the control group,while N-cad protein expression was lower than that in the control group. Conclusion Ga can inhibit the proliferation and migration of A549 and PC9 cells of lung adenocarcinoma in vitro,possibly by inhibiting aerobic glycolysis and epithelial-mesenchymal transition.