〔Abstract〕 Collagen antibody-induced arthritis(CAIA) is a novel mouse model of arthritis. Compared with collagen-induced arthritis(CIA) model, CAIA does not require more than 7 weeks to develop arthritis phenotype, and has a better induction effect for CIA model insensitive strains. However, the high dose inducers reported in previous literatures, on the one hand, caused serious adverse reactions and even death in mice, and on the other hand, resulted in unnecessary waste of 5-clone collagen antibodies cocktail. Reducing the dosage of 5-clone antibody cocktail and lipopolysaccharide(LPS) effectively induced the arthritis model of C57 BL/6 mice while avoiding the death of the model mice and saving the cocktail of 5-clone antibody, thus providing an improved method for the future research on the pathogenesis of RA.