〔Abstract〕 Objective To investigate the effect of lncRNA(DGUOK-AS1) on proliferation and apoptosis of lung squamous cell carcinoma. Methods LncRNA DGUOK-AS1 was found to be different in tumor tissues and normal tissues by TCGA database.SiRNA interference technology was used to decrease the expression of DGUOK-AS1 in lung squamous cell lines NCI-H520 and SK-MES-1. Cell proliferation was detected by CCK-8 method, and flow cytometry was used to detect changes in cell apoptosis, then Western blot was used to detect related proteins changes in cell proliferation, apoptosis and Wnt/β-Cantenin pathway. Results Compared with human normal lung epithelial cells(BEAS-2 B),the expression of DGUOK-AS1 increased in lung squamous cell carcinoma NCI-H520 and SK-MES-1 cells(P<0.001). The results of CCK-8 assay showed that decreased DGUOK-AS1 expression in NCI-H520 and SK-MES-1 could inhibit the proliferation ability of the cells compared with the negative control(P<0.01). Flow cytometry apoptosis detection results showed that reduced expression of DGUOK-AS1 could promote apoptosis with the negative control in NCI-H520 and SK-MES-1 cells(P<0.01).The results of Western blot showed that decreased expression of DGUOK-AS1 could reduce the expression of PCNA, Bcl-2, β-Cantenin, c-myc and Cyclin D1 proteins, and increase the expression of Bax proteins. RNA plasmid separation experiments showed that DGUOK-AS1 was mainly located in the cytoplasm. Conclusion The expression of long non-coding RNA DGUOK-AS1 increased in lung squamous cell carcinoma cells. The highly expressed DGUOK-AS1 may promote cell proliferation and inhibit cell apoptosis by affecting the Wnt/β-Cantenin pathway, and DGUOK-AS1 may be an important molecular target for the treatment of lung squamous cell carcinoma.