〔Abstract〕 To establish a C57 BL/6 J mice model of diarrhea-predominant irritable bowel syndrome(D-IBS) induced by wrap restrain stress(WRS), and to evaluate the feasibility and characteristics of this model. The D-IBS model of C57 BL/6 J mice was established by WRS. The general condition, fecal water content, body weight and intestinal transport function of mice were observed, and the pathological changes of colon were observed. Compared with the control group, the fecal water content in the D-IBS model group increased, the body weight decreased, the intestinal transport speed accelerated, and the colon length shortened. The colonic mucosa of mice in the model group showed inflammatory changes involving the lamina propria and mainly lymphocytosis. The D-IBS animal model of C57 BL/6 J mice can be prepared by WRS, and the symptoms and histopathological changes of intestinal mucosa in mice molded are more similar to those in human patient with D-IBS.