〔Abstract〕 Objective To establish a rapid detection method of fluorescent RT-RAA for novel coronavirus(SARS-CoV-2). Methods Using SARS-CoV-2 RNA transcribedin vitroas template,orf1 abgene andSgene were amplified rapidly by single strand DNA binding protein, recombinant enzyme and DNA polymerase at constant temperature of 40.5 ℃. Pharyngeal swabs from patients with known COVID-19 nucleic acid positive and other respiratory virus infections were used for preliminary evaluation. Results The method established in this research took less than 20 minutes to detect the two SARS-CoV-2 genes separately. The sensitivity of the two genes was 2 copies per reaction tube, and the specificity was 100%. The lowest detection limit of two primers was 100%. The peak time of the amplification reaction of the three repeated experiments was the same, and the shape of the curve was similar. Conclusion The method established in this study has high sensitivity, specificity and good reproducibility. The test results of clinical specimens have a high coincidence rate and do not require expensive equipment, so this method is suitable for rapid detection on site.