〔Abstract〕 Objective To study the inhibitory effect of forsythoside A(FA) on the inflammation induced by ischemia/reperfusion in PC12 cells. Methods Oxygen glucose deprivation/reoxygenation(OGD/R) model was established in PC12 cells, and PC12 cells were treated with different concentrations of FA(1.25, 2.5 and 5 μmol/L) to determine inflammatory factors(IL-1β, TNF-α and IL-6) level, Western blot was used to determine the expression of toll-like receptor-4(BTLR4);nuclear factor kappa(NF-κB) pathway-related proteins, and the mechanism was verified using lentiviral activation particles. Results FA could significantly inhibit the release of inflammatory factors, inhibit the protein expression of TLR4 and NF-κB p65, and also inhibit the expression of NF-κB p65 in the nucleus. TLR4 and NF-κB lentiviral activation particles were used to verify that FA regulated NF-κB p65 nuclear transfer through TLR4, thereby inhibiting the release of inflammatory factors and reducing inflammation. Conclusion FA can inhibit the expression of inflammatory factors by inhibition of NF-κB p65 nuclear translocation regulated by TLR4, so as to reduce the inflammatory response induced by ischemia-reperfusion and show brain protection effects.