〔Abstract〕 Objective To observe the effect of silencing prostate transmembrane protein, androgen induced 1(PMEPA1) on proliferation and migration of HepG2 hepatocellular carcinoma(HCC) cells. Methods qRT-PCR, immunohistochemistry, and Western blot were used to detect the mRNA and protein expression of PMEPA1 in HepG2 HCC cells. Besides, the relationship between the expression of PMEPA1 in liver cancer and its clinicopathological features was analyzed. Additionally, siRNA was employed to interfere with the expression of PMEPA1 in HepG2 HCC cells. MTT and Transwell methods were adopted to detect the proliferation and migration of HepG2 HCC cells. Results The expression of PMEPA1 protein in liver cancer was higher than that in normal liver, which was correlated with tumour size(P=0.036 6) and lymph node metastasis(P=0.035 8). Meanwhile, it had no correlation with gender, age, AFP level, vascular infiltration, histological grade, and TNM stage of HCC patients. Moreover, the PMEPA1 protein and mRNA expression of PMEPA1-siRNA HepG2 HCC cells was down-regulated(P<0.01), and the proliferation and migration of transfected cells in siPMEPA1 both decreased(P<0.01). Conclusion The expression of PMEPA1 may promote the proliferation and migration of hepatocellular carcinoma cells.