〔Abstract〕 Objective To explore the effect of the competitive binding of LncCCAT1 to miR-583 p on the stem-like characteristics of MCF-7 cells by regulating the expression of MAPK13. Methods Breast cancer tumor stem cells were isolated from MCF-7 cells and the expressions of CCAT1 and miR-583 p were detected by q-PCR in Adherent cells, suspension Spheres and Re-adherent cells. Expression of miR-583 p was measured after transfection of shRNA-CCAT1-2 and miR-583 p inhibitor into MCF-7 cells alone or in combination. The luciferase reporting experiment verified the targeting relationship between miR-583 p and CCAT1. The cells were divided into four groups: Control, shRNA-CCAT1-2, miR-583 p inhibitor and shRNA-CCAT1-2+miR-583 p inhibitor. ALDEFLUOR analysis was used to detect ALDH activity. The expression levels of SOX2, NANOG and ALDH1 were detected by Western blot. The pellet diameter was measured by pellet formation experiment. The lentivirus LV-MAPK13 was constructed and infected with MCF-7 cells to overexpress MAPK13. The MAPK13 level was detected by q-PCR, and the cells were divided into four groups: Control, shRNA-CCAT1-2, LV-MAPK13 and shRNA-CCAT1-2+LV-MAPK13 groups. The protein Western blot was used to detect the protein expression levels of MAPK13, SOX2, NANOG and ALDH1. Results Results showed that compared with Adherent group, CCAT1 expression levels increased(P<0.05)while miR-583 p expression levels decreased(P<0.05)in Spheres group. There was a direct targeting relationship between miR-583 p and CCAT1. Compared with the control group, the ALDH activity of shRNA-CCAT1-2 group decreased, the protein levels of SOX2, NANOG and ALDH1 decreased(P<0.05), and the ball diameter decreased(P<0.05). In the miR-583 p inhibitor group, ALDH activity increased, SOX2, NANOG, and ALDH1 protein levels increased(P<0.05), and ball diameter increased(P<0.05). Compared with the miR-583 p inhibitor group, the ALDH activity, SOX2, NANOG and ALDH1 protein levels and ball diameter of the shRNA-CCAT1-2+miR-583 p inhibitor group decreased(P<0.05). There was a direct targeting relationship between miR-583 p and MAPK13. Compared with Adherent group, MAPK13 levels of Spheres group increased(P<0.01). Compared with Control group, MAPK13 level of LV-MAPK13 group increased(P<0.001). Compared with the control group, MAPK13, SOX2, NANOG, and ALDH1 protein levels in shRNA-CCAT1-2 group decreased(P<0.05), while those in LV-MAPK13 group increased(P<0.05). Compared with LV-MAPK13 group, the protein levels of MAPK13, SOX2, NANOG and ALDH1 in shRNA-CCAT1-2+LV-MAPK13 group decreased(P<0.05). Conclusion LncCCAT1 competitively binds to miR-583 p to inhibit the stem cell like characteristics of MCF-7 cells by regulating the expression of MAPK13.